Ana iya amfani da PCR na CFX96Touch fluorescent quantitative a fannonin bincike iri-iri na ƙididdige acid nucleic, nazarin matakin magana, gano maye gurbi, gano GMO, da takamaiman samfurin.
Muhallin Aiki:
1.1 Yanayin aiki: 5-31 ° C
1.2 Aiki da zafi: dangi zafi ≤80%
1.3 Ikon Aiki: 100-240 VAC, 50-60Hz
Ayyuka da buƙatun fasaha na CFX96Touch fluorescence ƙididdige PCR
3.1 Babban aikin (* shine alamar da dole ne a hadu)
* 3.1.1 Tashoshi guda shida na gwaji, 5% PCR za a iya gane, kuma ana iya gano kwayoyin halitta guda 5 a lokaci guda, kuma ana gano tashar gano FRET na musamman a lokaci guda.
* 3.1.2 Tare da aikin PCR mai ƙarfi na zafin jiki, zaku iya gudanar da yanayin zafi daban-daban guda 8 a lokaci guda, kowane yanayin zafin jiki.
3.1.3 Bude cikakken reagents, daban-daban bincike da kuma asibiti reagents shafi;
3.1.4 Ya dace da hanyoyi masu haske iri-iri kamar Taqman, Molecular Beacon, Fret probe, Sybr Green i, da dai sauransu;
3.1.5 Buɗe, 0.2ml guda bututu, octal, farantin rijiyar 96, da sauransu.
* 3.1.6 na iya yin aiki da kansa, aiki na kan layi da gaske, babu buƙatar haɗa kwamfutar don saka idanu da girman girman girman PCR a ainihin lokacin;
3.2 Babban buƙatun fasaha (* shine alamar da dole ne a cika)
* 3.2.1 Samfurin iya aiki: 96 × 0.2ml, daidaitattun ƙayyadaddun ƙayyadaddun 96-rijiyar faranti (12 × 8) ana iya amfani da su;
3.2.2 Supplies Type: 0.2ml guda tube, takwas interlocking, 96-riji faranti, da dai sauransu.
3.2.3 Tsarin amsawa: 1-50μL (shawarar 10-25 μL);
* 3.2.4 Haske mai haske: LEDs shida tare da masu tacewa;
* 3.2.5 Mai ganowa: diodes masu ɗaukar hoto guda shida tare da masu tacewa;
* Gudun sanyaya lita 3.2.6: 5 ° C / s;
3.2.7 Yanayin sarrafa zafin jiki: 0 -100 ° C;
3.2.8 Daidaita Yanayin Zazzabi: ± 0.2 ° C (90 ˚C);
3.2.9 Daidaitaccen yanayin zafi: ± 0.4 ° C (90 ˚C cikin dakika 10);
* 3.2.10 Dynamic Temperature Gradient Aiki: Gudanar da yanayin zafi 8 daban-daban a lokaci guda; Tsarin kula da zafin jiki na gradient: 30 -100 ° C; kewayon bambancin zafin jiki na gradient: 1 - 24 ° C; gradient zafin jiki shiryawa lokaci: guda;
3.2.11 tashin hankali / watsi da tsayin raƙuman ruwa: 450-730 nm;
3.2.12 Hankali: Za a iya gano kwayar halitta guda ɗaya a cikin kwayoyin halittar ɗan adam;
3.2.13 Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwararren Ƙwaƙwalwa na Ƙaƙwalwa na Ƙaƙwalwa ) 10 yayi;
3.2.14 Nuni: 8.5 inch launi tabawa;
3.2.15 Yanayin Bincike na Data: daidaitaccen abu mai yawa, Melting Curve, CTBEENSICTICICAYSIRSICEDSICEDSECTION, Ma'adin GENITEDSICION, SELDICI NA GASKIYA, SYEDIT AREP ;
3.2.16 Export Data: Excel, Word, ko PowerPoint. Rahoton mai amfani ya ƙunshi saitunan gudu, zane-zane, da sakamakon bayanan tebur, waɗanda za'a iya bugawa ko adana su azaman PDF;
* 3.2.17 Nazarin tsarin Chromosomal: Hanya na ƙididdigar ƙididdiga na tsarin chromatin ta hanyar kwatancen rawar da ke tattare da lalata DNA ta hanyar kwatankwacin rawar DNA na genomic. Da gaske yana tabbatar da daidaituwar tsayi tsakanin tsarin chromatin da maganganun kwayoyin halitta;